Review



mmcp6  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    R&D Systems mmcp6
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mmcp6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmcp6/product/R&D Systems
    Average 93 stars, based on 10 article reviews
    mmcp6 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation"

    Article Title: Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25115738

    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and mMCP6+ MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Figure Legend Snippet: Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and mMCP6+ MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.

    Techniques Used: Expressing, Avidin-Biotin Assay, Marker, Staining, Immunohistochemical staining, Labeling, Derivative Assay

    SP induces mMCP4 mRNA in the presence of Chrna7 in cultured MCs. ( a , b ) qRTPCR analysis of mMCP4 and mMCP6 mRNA expression in primary cultures of peritoneal MCs from Wt and α7-KO mice. Individual treatments are mentioned at the X axis of the graphs. Gene expressions were normalized to the housekeeping gene TBP. Data represent the averages of at least 3 different experiments. * = p ≤ 0.05. Abbr.: α7-KO = Chrna7 knockout; mMCP = murine mast cell protease; SLURP1 = Ly-6/uPAR-related protein 1; SP = substance P; Wt = wild type.
    Figure Legend Snippet: SP induces mMCP4 mRNA in the presence of Chrna7 in cultured MCs. ( a , b ) qRTPCR analysis of mMCP4 and mMCP6 mRNA expression in primary cultures of peritoneal MCs from Wt and α7-KO mice. Individual treatments are mentioned at the X axis of the graphs. Gene expressions were normalized to the housekeeping gene TBP. Data represent the averages of at least 3 different experiments. * = p ≤ 0.05. Abbr.: α7-KO = Chrna7 knockout; mMCP = murine mast cell protease; SLURP1 = Ly-6/uPAR-related protein 1; SP = substance P; Wt = wild type.

    Techniques Used: Cell Culture, Expressing, Knock-Out

    Primers and probes used for qRTPCR.
    Figure Legend Snippet: Primers and probes used for qRTPCR.

    Techniques Used: Sequencing



    Similar Products

    mmcp6  (R&D Systems)
    93
    R&D Systems mmcp6
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mmcp6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmcp6/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    mmcp6 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    mast cell protease 4 conditional knockout mouse model  (Cyagen Biosciences)
    90
    Cyagen Biosciences mast cell protease 4 conditional knockout mouse model
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mast Cell Protease 4 Conditional Knockout Mouse Model, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mast cell protease 4 conditional knockout mouse model/product/Cyagen Biosciences
    Average 90 stars, based on 1 article reviews
    mast cell protease 4 conditional knockout mouse model - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    mouse mast cell protease 1 (mmcp-1)  (MultiSciences Biotech Co Ltd)
    90
    MultiSciences Biotech Co Ltd mouse mast cell protease 1 (mmcp-1)
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mouse Mast Cell Protease 1 (Mmcp 1), supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse mast cell protease 1 (mmcp-1)/product/MultiSciences Biotech Co Ltd
    Average 90 stars, based on 1 article reviews
    mouse mast cell protease 1 (mmcp-1) - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    mcpt6 antibody mab3736  (R&D Systems)
    93
    R&D Systems mcpt6 antibody mab3736
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mcpt6 Antibody Mab3736, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcpt6 antibody mab3736/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    mcpt6 antibody mab3736 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier
    mouse mast cell protease  (R&D Systems)
    94
    R&D Systems mouse mast cell protease
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mouse Mast Cell Protease, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse mast cell protease/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    mouse mast cell protease - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    elisa kits of mouse mast cell protease-1  (Dakewe Biotech Co)
    90
    Dakewe Biotech Co elisa kits of mouse mast cell protease-1
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Elisa Kits Of Mouse Mast Cell Protease 1, supplied by Dakewe Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits of mouse mast cell protease-1/product/Dakewe Biotech Co
    Average 90 stars, based on 1 article reviews
    elisa kits of mouse mast cell protease-1 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    goat antitryptase mcp 6  (R&D Systems)
    94
    R&D Systems goat antitryptase mcp 6
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Goat Antitryptase Mcp 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antitryptase mcp 6/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    goat antitryptase mcp 6 - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    anti mmcp6  (R&D Systems)
    94
    R&D Systems anti mmcp6
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Anti Mmcp6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mmcp6/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    anti mmcp6 - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    mast cell proteinase 1  (R&D Systems)
    93
    R&D Systems mast cell proteinase 1
    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and <t>mMCP6+</t> MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.
    Mast Cell Proteinase 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mast cell proteinase 1/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    mast cell proteinase 1 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and mMCP6+ MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.

    Journal: International Journal of Molecular Sciences

    Article Title: Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation

    doi: 10.3390/ijms25115738

    Figure Lengend Snippet: Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals ( n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for ( a , d ) mMCP4+ and mMCP6+ MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. ( c , f ) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in ( a , d )). ( b , e ) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress.

    Article Snippet: The primary antibodies used in this study are c-Kit (1:100, Acris Antibodies, Herford, Germany), mMCP4 (1:400, Biozol, Eching, Germany), and mMCP6 (1:800, AF3736, R&D Systems, Minneapolis, MN, USA).

    Techniques: Expressing, Avidin-Biotin Assay, Marker, Staining, Immunohistochemical staining, Labeling, Derivative Assay

    SP induces mMCP4 mRNA in the presence of Chrna7 in cultured MCs. ( a , b ) qRTPCR analysis of mMCP4 and mMCP6 mRNA expression in primary cultures of peritoneal MCs from Wt and α7-KO mice. Individual treatments are mentioned at the X axis of the graphs. Gene expressions were normalized to the housekeeping gene TBP. Data represent the averages of at least 3 different experiments. * = p ≤ 0.05. Abbr.: α7-KO = Chrna7 knockout; mMCP = murine mast cell protease; SLURP1 = Ly-6/uPAR-related protein 1; SP = substance P; Wt = wild type.

    Journal: International Journal of Molecular Sciences

    Article Title: Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation

    doi: 10.3390/ijms25115738

    Figure Lengend Snippet: SP induces mMCP4 mRNA in the presence of Chrna7 in cultured MCs. ( a , b ) qRTPCR analysis of mMCP4 and mMCP6 mRNA expression in primary cultures of peritoneal MCs from Wt and α7-KO mice. Individual treatments are mentioned at the X axis of the graphs. Gene expressions were normalized to the housekeeping gene TBP. Data represent the averages of at least 3 different experiments. * = p ≤ 0.05. Abbr.: α7-KO = Chrna7 knockout; mMCP = murine mast cell protease; SLURP1 = Ly-6/uPAR-related protein 1; SP = substance P; Wt = wild type.

    Article Snippet: The primary antibodies used in this study are c-Kit (1:100, Acris Antibodies, Herford, Germany), mMCP4 (1:400, Biozol, Eching, Germany), and mMCP6 (1:800, AF3736, R&D Systems, Minneapolis, MN, USA).

    Techniques: Cell Culture, Expressing, Knock-Out

    Primers and probes used for qRTPCR.

    Journal: International Journal of Molecular Sciences

    Article Title: Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation

    doi: 10.3390/ijms25115738

    Figure Lengend Snippet: Primers and probes used for qRTPCR.

    Article Snippet: The primary antibodies used in this study are c-Kit (1:100, Acris Antibodies, Herford, Germany), mMCP4 (1:400, Biozol, Eching, Germany), and mMCP6 (1:800, AF3736, R&D Systems, Minneapolis, MN, USA).

    Techniques: Sequencing